6*His Monoclonal Antibody

Conjugate: Non-conjugated. Clonality: Monoclonal. Form: Liquid. Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Product Type: Tag/Control Antibodies. Clone Number: 3G5H8. Aliases: 6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag. Immunogen: HHHHHH(6x His) synthetic peptide conjugate to KLH. Raised in: Mouse. Species Reactivity: All. Tested Applications: ELISA, WB; Recommended dilution: WB:1:500-1:2000. Background: A His tag is a tag of 6 histidines added to the end of the protein that the DNA codes for, so the DNA with contain 6 repeats of the His codon. What it is used for is purifying the protein that the DNA codes for. Histidines are positively charged amino acids and a group of 6 of them allows the protein to bind to a negatively charged column. Histidines are also special in that they have a pKa around 7, meaning that once bound to the column they can easily be unbound. Isotype: IgG2a. Purification Method: >95%, Protein A purified. Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. Target Names: 6*His.