Anti-V5 Tag, Clonility V5.E10
Price range: $131.25 through $237.30
| Size |
50 µg ,100 µg |
|---|
General Description
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Source protein: Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Protein A affinity chromatography purified protein from mouse ascites fluid
Clonality and Host Species:Â Â V5.E10, Mouse
Immunogen and Isotype:Â Â Â Â GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH, IgG2a
Specificity and reactivity:Â Â Â Â Detects V5 -tagged fusion proteins.
Application:Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Western blot, Dot blot, ELISA, IHC/IS. Western blot (with ECL) 1-5 ng of V5-tagged fusion protein.
Available form:                       50 µg of lyophilized antibody (lyophilized in 10mM PBS, pH 7.2 with 1% BSA and 0.05% Sodium azide; NaN3)
Working procedure:Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Western blot (with ECL) 1:1000-3,000 dilution, incubate for 1 hour at room temperature. For the best results, determine the optimal working antibody dilution for your protein of interest.
Storage:                                    Supplied lyophilized antibodies can be stored at -20ºC for 3 years. Reconstitute the lyophilized antibody with distilled water to 1mg/ml or a desired concentration before use. Reconstituted liquid can be stored at -20ºC for 2 years. Centrifuge after first thaw to maximum product recovery. Aliquot into smaller volume to avoid repeated freeze-thaw cycles. Store aliquots at 4ºC for several days to 2 weeks.
MAECENAS IACULIS
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ADIPISCING CONVALLIS BULUM
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