Proteinase K, Lyophilized

$163.23 – $654.06

Size

100 mg

500 mg

Description

General Description

Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.

Proteinase K is typically used at a concentration of 50-100 μg/ml. It is active with or without the presence of SDS, urea, EDTA or various metal ions, but the activity of proteinase K can be increased by adding the denaturing agents and the structure of proteinase K can be stabilized by addition of Ca 2+. Proteinase K is inactivated by heating to 95°C for 10 minutes or using an inhibitor such as PMSF, AEBSF or DFP.

Product Specifications: Proteinase K, RNase/DNase free MOLECULAR BIOLOGY GRADE

Molecular Weight: 28.5 kDa

Activity: >= 30 U/mg

Unit Definition: One unit is defined as the amount of enzyme that will liberate 1.0 μmol tyrosine (Folin-positive amino acid) from casein per minute at 37°C, pH 7.5.

Storage / Handling: Store at -20°C.

Reconstitute in 50mM Tris-HCl (pH 8.0), 3mM CaCl2.

Manual

Safety Datasheet

[vc_row][vc_column][vcex_button url=”`{`acf field=“safety“`}`” size=”large” text_source=”custom_text” aria_label=”Safety Datasheet” target=”blank” icon_left=”ticon ticon-file-pdf-o” icon_left_padding=”10px”]Safety Datasheet[/vcex_button][/vc_column][/vc_row]

Certificate of Analysis

Shipping & Delivery

MAECENAS IACULIS

Vestibulum curae torquent diam diam commodo parturient penatibus nunc dui adipiscing convallis bulum parturient suspendisse parturient a.Parturient in parturient scelerisque nibh lectus quam a natoque adipiscing a vestibulum hendrerit et pharetra fames nunc natoque dui.

ADIPISCING CONVALLIS BULUM

Vestibulum penatibus nunc dui adipiscing convallis bulum parturient suspendisse.
Abitur parturient praesent lectus quam a natoque adipiscing a vestibulum hendre.
Diam parturient dictumst parturient scelerisque nibh lectus.

Scelerisque adipiscing bibendum sem vestibulum et in a a a purus lectus faucibus lobortis tincidunt purus lectus nisl class eros.Condimentum a et ullamcorper dictumst mus et tristique elementum nam inceptos hac parturient scelerisque vestibulum amet elit ut volutpat.