Protease Inhibitor Cocktail [100X], General-EDTA Free

Our Protease Inhibitor Cocktail, General-EDTA Free [100X] contains optimized concentration of protease inhibitors such as AEBSF, Aprotinin, E-64 [N-(trans-Epoxysuccinyl)-L-leucine 4-guanidinobutylamide], Bestatin, Leupeptin for broad spectrum inhibition of proteases that target serine proteases (e.g., trypsin, chymotrypsin, plasmin, kallikrein and thrombin), cysteine proteases (e.g., calpain, papain, cathepsin B, and cathepsin L), and aminopeptidases (e.g., leucine aminopeptidase and alanyl aminopeptidase), etc.

The tissue and cell extracts contain a number of endogenous proteases, that are capable of modifying the proteins present in the extract. The need of protease inhibitors arises to protect the proteins from damage caused by the proteases. Moreover, to improve the yield of native proteins, use of protease inhibitor cocktail as well as phosphatase and other inhibitors is recommended during the extraction process. Our Protease Inhibitor Cocktail-General provides complete protection of proteins from degradation by endogenous proteases that may be released during extraction and purification of proteins from animal and plant tissues, cultured cells, yeast or bacteria.

The inhibitor cocktail is supplied as a ready to use solution at 100X concentration, which makes it easier to use in low volume at 1X, 2X or more for samples with high protease activities and is better or equivalent in performance when compared with Roche tablet and Sigma’s protease inhibitor cocktails. Our inhibitor cocktail helps in preserving the proteins from degradation by proteases and used during the extraction of proteins from different tissues and cells.