Biotin-PEG2-Amine

General Description

Biotin-PEG2-Amine is a water-soluble biotin compound containing a polyethylene glycol (PEG) spacer arm and a terminal primary amine for conjugation via EDC and other crosslinker methods. The amine group can be coupled to carboxyl groups or 5′-phosphate groups to form stable amide bonds. The hydrophilic PEG spacer increases solubility in aqueous media and increases membrane impermeability of the molecules conjugated to the biotin compound. It also helps to minimize steric hindrance involved with the binding to avidin molecules.

Chemical Name:        (+)-Biotinyl-3,6-dioxaoctanediamine

Molecular Formula:   C₁₆H₃₀N₄O₄S

Molecular Weight:      374.50

CAS NO.:                      138529-46-1

Purity:                           98%

Spacer arm length:     20.4Å

Form:                             White to off-white solid

Solubility:                     ~25mg/ml in water

Storage:                         4⁰C

Features of Biotin-PEG2-Amine:

• Biotinylation— label molecules and surfaces for assays or affinity purification methods involving avidin or streptavidin probes and resins

• Amine-activated— primary amine can be crosslinked to proteins and material surfaces using EDC and other crosslinkers

• Pegylated— polyethylene glycol (PEG) groups in spacer arm enhances water solubility of biotinylated molecules

• Medium length— spacer arm (total length added to target) is 20.4 Å