Anti-ERK1/2, Clonility E31R

Source protein:           

Protein A affinity
chromatography purified protein from mouse ascites fluid.

Clonality and Host Species:    

E31R, Mouse

Immunogen and Isotype:        

Recombinant full length
ERK1 protein, IgG1

Specificity and reactivity:       

Detects the ERK1 and
ERK2 protein (e.g. from human and mouse cells).

Application:                              

IP, Western blot

Available form:                        

Lyophilized 1mg/mL (lyophilized
in 10mM PBS, pH 7.2 with 1% BSA and 0.05% Sodium azide; NaN₃)

                                  
Working procedure:                

Western blot (with ECL)
1:1000 dilution incubates for 1 hour at room temperature. For the best results,
determine the optimal working antibody dilution for your protein of interest.

Storage:                                

Supplied lyophilized
antibodies can be stored at -20⁰C for 3 years.

Reconstitute the
lyophilized antibody with distilled water to 1mg/ml or a desired concentration
before use.  

Reconstituted liquid can be
stored at -20^oC for 2 years. Centrifuge after first thaw to maximum
product recovery. Aliquot into smaller volume to avoid repeated freeze-thaw
cycles.  Store aliquots at 4⁰C
for several days to 2 weeks.