Anti-GFP Tag, Clonility GF28R

Source protein:           

Protein A affinity chromatography purified protein from mouse ascites fluid.

Clonality and Host Species:    

GF28R, Mouse

Immunogen and Isotype:        

GFP from jelly Aequorea Victoria (FLAG) synthetic N-terminal peptide conjugated to KLH, IgG2b

Specificity and reactivity:       

Detects the native and denatured forms of GFP and its variants such as EGFP, YEP, EYFP and CFP.

Application:                              

Western blot, Dot blot, ELISA, IHC/IS, Western blot (with ECL) 1-10 ng of GFP or GFP fusion protein.

Available form:                        

Lyophilized 1mg/mL (lyophilized in 10mM PBS, pH 7.2 with 1% BSA and 0.05% Sodium azide; NaN₃)

                                          
Working procedure:                

Western blot (with ECL) 1:1000-3,000 dilution incubate for 1 hour at room temperature. For the best results, determine the optimal working antibody dilution for your protein of interest.

Storage:                                

Supplied lyophilized antibodies can be stored at 20⁰C for 3 years.

Reconstitute the lyophilized antibody with distilled water to 1mg/ml or a desired concentration before use. 

Reconstituted liquid can be stored at -20^oC for 2 years. Centrifuge after first thaw to maximum product recovery. Aliquot into smaller volume to avoid repeated freeze-thaw cycles.  Store aliquots at 4⁰C for several days to 2 weeks.