Anti.HA Tag, Clonility HA.C5

General Description

Source protein:                          Protein A affinity chromatography purified protein from mouse ascites fluid.

Clonality and Host Species:    HA. C5, Mouse

Immunogen and Isotype:         YPYDVPDYA (HA) synthetic peptide conjugated to KLH, IgG3

Specificity and reactivity:        Detects the HA-tagged fusion proteins, regardless of the tag’s location in the fusion protein sequences (reacts with N-terminal, C-terminal or internal HA tagged fusion proteins)

Application:                                Western blot. Dot blot, ELISA, IHC/IS. Western blot (with ECL) 1-5 ng of HA-tagged fusion protein.

Available form:                          50 µg of lyophilized antibody (lyophilized in 10mM PBS, pH 7.2 with 1% BSA and 0.05% Sodium azide; NaN₃).

Working procedure:                  Western blot (with ECL) 1:1000-3,000 dilution incubates for 1 hour at room temperature. For the best results, determine the optimal working antibody dilution for your protein of interest.

Storage:                                       Supplied lyophilized antibodies can be stored at -20^ºC for 3 years. Reconstitute the lyophilized antibody with distilled water to 1mg/ml or a desired concentration before use. Reconstituted liquid can be stored at -20^ºC for 2 years. Centrifuge after first thaw to maximum product recovery. Aliquot into smaller volume to avoid repeated freeze-thaw cycles.  Store aliquots at 4^ºC for several days to 2 weeks.