Anti-V5 Tag, Clonility V5.E10

$131.25$237.30

Size

50 µg

,

100 µg

SKU: 10007 Categories: ,
Description

General Description

 

Source protein:                         Protein A affinity chromatography purified protein from mouse ascites fluid

Clonality and Host Species:   V5.E10, Mouse

Immunogen and Isotype:        GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH, IgG2a

Specificity and reactivity:       Detects V5 -tagged fusion proteins.

Application:                               Western blot, Dot blot, ELISA, IHC/IS. Western blot (with ECL) 1-5 ng of V5-tagged fusion protein.

Available form:                         50 µg of lyophilized antibody (lyophilized in 10mM PBS, pH 7.2 with 1% BSA and 0.05% Sodium azide; NaN3)

Working procedure:                 Western blot (with ECL) 1:1000-3,000 dilution, incubate for 1 hour at room temperature. For the best results, determine the optimal working antibody dilution for your protein of interest.

Storage:                                     Supplied lyophilized antibodies can be stored at -20ºC for 3 years. Reconstitute the lyophilized antibody with distilled water to 1mg/ml or a desired concentration before use. Reconstituted liquid can be stored at -20ºC for 2 years. Centrifuge after first thaw to maximum product recovery. Aliquot into smaller volume to avoid repeated freeze-thaw cycles.  Store aliquots at 4ºC for several days to 2 weeks.

 

 

Manual
Safety Datasheet

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Certificate of Analysis

Certificate of Analysis

Shipping & Delivery

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