Biotin-BMMCC

General Description

BMCC-Biotin is membrane non-permeable and water insoluble. BMCC-Biotin is useful for labeling protein cysteines and other molecules that contain sulfhydryl groups. This reagent specifically
reacts with reduced thiols (-SH) in near-neutral buffers to form irreversible thioether bonds. The unique feature of BMCC-Biotin is its spacer arm cyclohexane ring; this has a stabilizing effect that minimizes hydrolysis and degradation of the maleimide group until it has the opportunity to conjugate with target thiols.

Full Name:                     1-Biotinamido-4-[4´-(maleimidomethyl)cyclohexanecarboxamido]butane 

Size:                                50 mg

Molecular Formula:     C26H39N5O5S

Molecular Weight:        533.68

Spacer Arm Length:     32.6Å

Solubility:                      ~4.5mg/mL (8.5mM) in DMSO.  May require heating at 37°C for up to 10 minutes with intermittent mixing.

Storage:                           Shipped at ambient temperature. Upon receipt, store desiccated at 4°C.

Features/ Benefits:
• Protein labeling—biotinylate antibodies or other proteins for use in protein methods

• Membrane-permeable—can be used to label intracellularly

• Thiol-reactive— reacts with sulfhydryls (-SH) group, e.g. the side-chain of cysteine (C)

• Maleimide-activated— perform reactions at pH 6.5 to 7.5 in buffers such as PBS

• Irreversible— forms permanent thioether bonds; spacer arm cannot be cleaved

• Medium length—spacer arm (total length added to target) is 32.6 angstroms; contains cyclohexane ring, which stabilizes adjacent maleimide