Caspase-3, 7 Substrate (Ac-DEVD-AFC) [1mM], Fluorometric

$185.22

Size

250µl

Description

Caspase-3, 7 Substrate (Ac-DEVD-AFC) [1mM], Fluorometric

General description

This product is a ready-to-use fluorometric substrate for Caspase-3/CPP32 (Km = 9.7 µM) and related caspases that recognize the amino acid sequence DEVD. The sequence DEVD is based on Caspase-3 cleavage site in poly (ADP-ribose) polymerase (PARP). CPP32 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate DEVD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or a multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.

Synonyms: Ac-DEVD-AFC, Caspase-3 Substrate Fluorogenic

Peptide Sequence: Ac-Asp-Glu-Val-Asp-AFC

Appearance: Liquid

Formulation: DMSO (1 mM)

Molecular Formula: C₃₀H₃₃F₃N₅O₁₃

Molecular Weight: 729

Solubility: DMSO

InChi Key: GZDRODOYEFEHGG-NUDCOPPTSA-N

PubChem CID: 644234

Handling: Protect from light and moisture

Storage temp.: -20°C

Manual

Safety Datasheet

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Certificate of Analysis

Shipping & Delivery

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