Caspase-4 Substrate (Ac-LEVD-AFC) [1mM], Fluorometric

Caspase-4 Substrate (Ac-LEVD-AFC) [1mM], Fluorometric

General description

This product is a ready-to-use fluorometric substrate for Caspase-4 and related caspases that recognize the amino acid sequence LEVD. Caspase-4 and related caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate LEVD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.

Synonyms:                        Ac-LEVD-AFC, Caspase-4 Substrate, Fluorogenic

Peptide Sequence:          Ac-Leu-Glu-Val-Asp-AFC

Appearance:                     Liquid

Formulation:                    DMSO (1 mM)

Molecular Formula:        C₃₂H₄₀F₃N₅O₁₁

Molecular Weight:          727.5

InChi Key:                         FETZKXHXYOHAAN-UHFFFAOYSA-N

PubChem CID:                 44135103

Handling:                          Protect from light and moisture

Storage temp.:                 -20°C