Caspase-8 Substrate (Ac-IETD-AFC) [1mM], Fluorometric

$156.28

Size

250µl

Description

Caspase-8 Substrate (Ac-IETD-AFC) [1mM], Fluorometric

General description

This product is a ready-to-use fluorometric substrate for FLICE/Caspase-8 and related caspases that recognize the amino acid sequence IETD. The sequence IETD is based on Caspase-8 cleavage site in CPP32/Caspase-3 proenzyme. FLICE and related caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate IETD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.

Synonyms: Ac-IETD-AFC, Caspase-8 Substrate, Fluorogenic

Peptide Sequence: Ac-Ile-Glu-Thr-Asp-AFC

Appearance: Liquid

Formulation: DMSO (2 mM)

Molecular Formula: C₃₁H₃₈F₃N₅O₁₂

Molecular Weight: 729.7

InChi Key: CGUOWXRXLQXOKO-FCQBACSVSA-N

PubChem CID: 25108570

Handling: Protect from light and moisture

Storage temp.: -20°C

Manual

Safety Datasheet

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Certificate of Analysis

Shipping & Delivery

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