EGS (Ethylene Glycol bis(succinimidyl succinate))

General Description

Ethylene glycolbis(succinimidylsuccinate), is a water insoluble homobifunctional crosslinker that must be dissolved in DMSO or DMF before using in an aqueous reaction. EGS crosslinking reacts through amine-reactive NHS esters at both ends. EGS crosslinker’s spacer arm is cleaved by hydroxylamine at pH 8.5 for 3-6 hours at 37°C. The EGS protein crosslinker possesses amino reactive NHS esters on both ends of the molecule and can be used to label intracellular proteins, since the molecule is cell membrane permeable. EGS crosslinker is suitable for ChIP assays to determine DNA-protein binding interactions.

Name:                         Ethylene Glycolbis(succinimidylsuccinate)

Synonyms:                 Ethylene glycol disuccinate di(N-succinimidyl) ester; EGS; Di(N-succinimidyl) ethylene glycol disuccinate; Ethylene glycol bis(succinimidyl succinate)

CAS Number:             70539-42-3

Molecular Weight:    660.47

Color & Form:            Solid white powder

Melting Point:            >130° C

Solubility:                   DMF (Dimethylformamide), DMSO (Dimethylsulfoxide)

Features of the EGS crosslinker:

• Reactive groups: NHS ester (both ends)
• Reactive towards: amino groups (primary amines)
• Water-insoluble: dissolve first in DMF or DMSO, then dilute in water
• Membrane-permeable, allowing for intracellular crosslinking
• After reversible crosslinking with EGS, Lactate dehydrogenase retained 60% of its activity