Imidazole [2M] Solution, pH7.5

Imidazole is a critical buffer substance for bioprocesses to enhance the purity of the target protein. Its most widely used application is the preparation of buffer solutions to operate downstream purification columns.

Imidazole [2M] Solution, pH 7.5 is useful in molecular biology, protein chemistry, biochemical applications and an excellent buffer at pH range of 6.2 to 7.8, 25 degrees C, often used in the enzymatic reactions.

The buffers containing Imidazole are commonly used for the purification of histidine-tagged proteins by immobilized metal affinity chromatography (IMAC). Imidazole is also a chelator for the binding of various divalent cations and can be used for the elution of histidine containing proteins from divalent cation resins. It is also used as a component of the buffer for assaying horseradish peroxidase (HRP), as a chelator for binding of many divalent cations and reverse staining of SDS-PAGE gels for detection of proteins.

Vapor Pressure:              0.003 hPa (20°C)

Autoignition temp.:        480°C

pH:                                    10.5 (20°C, 67 g/L in H2O)

Boiling Point:                   256°C/1013 hPa

Melting Point:                  90°C

Solubility:                         633 g/L

Density:                            1.233 g/cm3 at 20°C

Storage temp.:                Store at 5°C to 30°C

Product Citations:

1. Zhu, Liyuan et al (2023) Light-switchable transcription factors obtained by direct screening in mammalian cells. NATURE COMMUNICATIONS, http://doi.org/10.1038/s41467-023-38993-6