LDS Sample Buffer [4X]

General Description

LDS Sample Buffer (4X) is used to prepare protein samples for denaturing gel electrophoresis with Bis-Tris or Tris-Acetate gels.

The LDS Sample Buffer [4X] contains 988 mM Tris, 2.04 mM EDTA, 8 % LDS (Lithium dodecyl sulfate), 40 % Glycerol, 0.88 % Commassie Brilliant Blue G250, 0.7 mM Phenol red and doesn’t contain any reducing agent. Coomassie G250 gives a sharp dye front with both MES and MOPS SDS running buffers and migrates much closer to the moving ion front than bromophenol blue. Bromophenol blue runs more slowly than some peptides with MES SDS Running Buffer. This ensures that small peptides do not run off the gels.

Use with a protein reducing agent such DTT, TCEP (10 mM; use freshly prepared) or Beta mercaptoethanol (2%) to reduce disulfide bonds in the protein sample.