PFP-Biotin
$159.75
| Size |
50 mg |
|---|
General Description
PFP-biotin is a pentafluorophenyl-ester biotinylation reagent that reacts with both primary and secondary amines for biotinylation. PFP-Biotin has similar properties like NHS-biotin but is more reactive. It is water insoluble and must be first dissolved in a minimal amount of an organic solvent, such as dimethylsulfoxide (DMSO) or dimethylformamide (DMF) and then added to the aqueous buffer containing the protein or other material for biotinylation.
Molecular Formula:Â Â Â C16H15F5N2O3S
Molecular Weight:Â Â Â Â 410.36
Spacer Arm:Â Â Â Â Â Â Â Â Â 9.6 Ã…
Reactive Group:Â Â Â Â Â Â Pentafluorophenyl ester
Features of PFP-Biotin:
1. Membrane-permeable— It can be used to label inside cells (intracellular)
2. Amine-reactive— It reacts with primary and secondary amines of proteins and other molecules at pH 7.0 to 9.0
3. Irreversible— It forms permanent amide bonds; spacer arm cannot be cleaved
4. Solubility— Insoluble in water, must be dissolved in DMSO or DMF before further dilution in aqueous buffers
Very short spacer arm — total length added to target is 9.6 Å and consists of the native biotin valeric acid group only
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