Ponceau S Solution

General Description

The Ponceau S Solution is a widely used staining solution for proteins on Western blots and in sequencing methods. It offers fast and reversible staining of protein bands without affecting any blotted / sequenced polypeptides. Staining can be done in less than five minutes with quick de-staining.  

Ponceau S is the sodium salt of a diazo dye. Ponceau S solution is a suitable reagent for use in electrophoresis studies. The Ponceau S stain comes ready-to-use and is designed for rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. It may be employed as stain for the purified fibrinolytic enzyme (CK) for the analysis of N-terminal amino acid sequence of the first 14 residues of CK by a protein sequencer. Ponceau S staining on nitrocellulose and PVDF is reversible by washing the stained membrane with 0.1M NaOH for 1 minute. The stained and destained membrane can then be immunologically detected (Western blotting).