SIAB (N-Succinimidyl(4-iodoacetyl) Aminobenzoate)

General Description

SIAB is an amine- and sulfhydryl-reactive heterobifunctional crosslinker. It contains an amine-reactive N-hydroxysuccimide (NHS) ester and a sulfhyryl-reactive iodoacetyl group and useful for preparing enzyme conjugates or immunotoxins. SIAB must be first dissolved in an organic solvent, such as DMSO or DMF, and then added to the aqueous reaction mixture. SIAB is lipophilic, membrane-permeable and does not possess a charged group. NHS esters react with primary amino groups (-NH2) present on the side chain of lysine (K) residues and the N-terminus polypeptides. The reaction proceeds efficiently in pH 7-9 buffers to form stable amide bonds and results in the release of Nhydroxysuccinimide.

Molecular Weight:  402.14

Spacer Arm:             10.6 Å

Features of SIAB:

1. Reactive groups: NHS ester and iodoacetyl

2. Reactive toward: amino and sulfhydryl groups

3. Non-cleavable

4. Water-insoluble (dissolve first in DMF or DMSO)

5. Useful for preparing stable enzyme-IgG conjugates

6. Forms conjugates with liposomes