Sulfo SANPAH (Sulfosuccinimidyl 6-(4’-azido-2’-nitrophenylamino) hexanoate)

General description

Sulfo-SANPAH is a heterobifunctional crosslinker containing an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable nitrophenyl azide. NHS esters react efficiently with primary amino groups (-NH2) in pH 7-9 buffers to form stable amide bonds. On exposure to UV light, nitrophenyl azides form a nitrene group that can initiate addition reactions with double bonds, insertion into C-H and N-H sites, or subsequent ring expansion to react with a nucleophile (e.g., primary amines).

Features and Benefits:

• Reactive groups are Sulfo-NHS ester and nitrophenyl azide
• Reactive towards amino groups and any nucleophile
• Non-cleavable
• N-Sulfosuccinimidyl-6-(4′-azido-2′-nitrophenylamino) hexanoate
• Optimal photolysis occurs at 320-350 nm and minimizes damage to biomolecules by irradiation
• Water-soluble and non-cleavable