Tris-Acetate-EDTA (TAE) Buffer [50X]

General Description

Cepham Life Sciences’ 50X TAE (Tris-Acetate-EDTA) Buffer contains 2M Tris, 1M Acetic acid with 0.05M EDTA and is used for running electrophoresis of nucleic acids in the gels for providing electrical conductivity and maintaining the pH. The EDTA present in the buffer inhibits metal dependent nucleases by chelating the divalent cations (Ca2+, Mg2+), thereby protects the DNA from nucleases during the electrophoresis.

The buffer can be used for both genomic and large supercoiled DNA, and can also be used as a gel preparation buffer. This solution has a lower buffering capacity than TBE buffer, therefore, periodic replacement of the buffer during prolonged electrophoresis is recommended. However, dsDNA runs faster with TAE buffer than TBE. The 50X concentrated TAE buffer should be diluted with molecular biology grade water to 1X before running the electrophoresis.

Applications

• Electrophoresis of nucleic acids in agarose and polyacrylamide gels
• Recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA
• Can be used both as a running buffer and as a gel preparation buffer
• Filtered sterilized with a 0.22 µm membrane filter